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Hybridization of DNA probes to A. marginale isolates from different sources and detection in Dermacentor andersoni
R E Ambrosio1, E S Visser, Y Koekhoven
1Molecular Biology Section, Veterinary Research Institute, Onderstepoort.
Abstract:
DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to identical bands on all of these isolates. The hybridization patterns suggests that the Virginia, Florida and the South African isolates are similar. A number of bands were obtained with the Washington isolate which differed from those obtained with the other isolates. Probe AC-2 could be developed to identify relatedness among Anaplasma isolates. Probe AC-2 detected A. marginale DNA in midgut material from infected Dermacentor andersoni ticks. No hybridization was obtained with DNA from salivary gland tissues from these infected ticks.
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