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Exonuclease I-assisted fluorescence aptasensor for tetrodotoxin
Yifeng Lan1, Guojie Qin2, Yanli Wei1
1Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China.
Ecotoxicology and Environmental Safety
|March 16, 2020
Summary
A novel fluorescence aptasensor was developed for detecting tetrodotoxin (TTX). This highly sensitive method utilizes aptamers and berberine for accurate TTX quantification, offering a significant advancement in toxin detection.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Tetrodotoxin (TTX) is a potent neurotoxin.
- Accurate and sensitive detection of TTX is crucial for food safety and public health.
Purpose of the Study:
- To develop a highly specific and sensitive fluorescence aptasensor for tetrodotoxin determination.
- To establish a label-free sensing system for TTX detection.
Main Methods:
- Utilized tetrodotoxin-aptamer as the recognition element.
- Employed berberine as a signal reporter and exonuclease I for background signal elimination.
- Leveraged fluorescence quenching and enhancement mechanisms for TTX detection.
Main Results:
- The aptasensor demonstrated a linear response to tetrodotoxin concentrations ranging from 0.030 nM to 6.0 × 10^3 nM.
- Achieved a low detection limit of 11.0 pM, surpassing existing sensor methods.
- Exhibited high specificity and sensitivity in tetrodotoxin determination.
Conclusions:
- The developed fluorescence aptasensor offers an ultrasensitive and label-free method for tetrodotoxin detection.
- This approach provides a robust platform for monitoring tetrodotoxin contamination.
- The sensor's performance indicates its potential for practical applications in toxin analysis.
