Identifying alternative solvents for C₆₀ manufacturing using singular and combined toxicity assessments

Linseed oil, olive oil, and sunflower oil were selected based on green chemistry principles and C₆₀ solubility as alternative solvents to replace 1,2,4-trimethylbenzene (TMB) for C₆₀ manufacturing. Singular acute toxicity experiments of C₆₀ and the four solvents was performed using Daphnia magna to identify the solvent with the lowest toxicity and estimate the toxicity of C₆₀. The EC₅₀ for C₆₀ was estimated to be higher than 176 ppm. The toxicity of the solvents increased from sunflower oil to olive oil, linseed oil, and TMB. Combined toxicity tests were conducted to investigate the interaction between C₆₀ and the solvent since essential oils can be nanocarriers and facilitate the transport of C₆₀ into the cell membranes, which would increase its toxicity. Various concentrations of C₆₀ (0, 11, 22, 44, 88, and 176 mg/L) were mixed with solvents at their EC₅₀ concentrations. The toxicity of linseed oil increased with increasing C₆₀ concentrations. For olive and sunflower oil, the toxicity was lowered with low concentrations of C₆₀. Olive oil was determined to be a suitable solvent for C₆₀ manufacturing based on singular and combined toxicity assessments. This study showed the importance of considering combined toxicity for solvent selection.