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Fractal SERS nanoprobes for multiplexed quantitative gene profiling.
Xiwei Wang1, Bingyi Liu1, Mingshu Xiao1
1Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, PR China.
Biosensors & Bioelectronics
|March 17, 2020
Summary
Researchers developed a novel fractal SERS nanoprobe for ultrasensitive DNA and microRNA detection. This probe offers robust internal calibration and multiplexing for reliable biological sensing.
Area of Science:
- Nanotechnology
- Spectroscopy
- Biotechnology
Background:
- Quantitative analysis is crucial for biological and chemical sensing.
- Surface-enhanced Raman spectroscopy (SERS) faces challenges in achieving reliable quantitative measurements.
- Developing sensitive and reproducible SERS probes is essential for advanced diagnostics.
Purpose of the Study:
- To develop a novel fractal SERS nanoprobe for ultrasensitive detection of DNA and microRNA.
- To incorporate a robust internal calibration standard for accurate SERS quantification.
- To achieve high multiplexing capability for simultaneous detection of multiple nucleic acid targets.
Main Methods:
- Fabrication of a fractal SERS nanoprobe with a gold core, hollow gap, and tunable stellate outer shell.
- Embedding Raman tags within the hollow gap to serve as an internal calibration standard.
- Utilizing the tunable morphology for enhanced and reproducible SERS signals.
Main Results:
- Demonstrated ultrasensitive detection of nucleic acids down to the femtomolar level.
- Achieved reproducible quantitative measurements through the internal calibration standard.
- Showcased high multiplexing capability with crosstalk-free Raman tag encoding.
Conclusions:
- The developed fractal SERS nanoprobe offers a simple, reliable, and widely applicable solution for quantitative nucleic acid detection.
- The internal calibration standard effectively corrects for sample and measurement fluctuations.
- This SERS sensor is highly attractive for multiplexed bioassays, genomic screening, and diagnostic applications.
