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Related Experiment Videos

Arboviruses recovered from sentinel cattle using several virus isolation methods.

G P Gard1, R P Weir, S J Walsh

  • 1Division of Primary Production, Darwin, N.T., Australia.

Veterinary Microbiology
|October 1, 1988
PubMed
Summary

This study monitored arboviruses in cattle, identifying 14 virus types including bluetongue and EHD. Different cell culture systems proved most effective for isolating specific virus groups from sentinel steers.

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Area of Science:

  • Veterinary Virology
  • Arthropod-borne Virus Research
  • Animal Health Surveillance

Background:

  • Arboviruses pose a significant threat to cattle health.
  • Understanding arbovirus circulation in livestock is crucial for disease prevention.
  • Sentinel animal studies provide valuable insights into viral epidemiology.

Purpose of the Study:

  • To investigate the prevalence and diversity of arboviruses in cattle.
  • To evaluate the efficacy of different diagnostic systems for arbovirus isolation.
  • To identify specific arbovirus groups circulating in sentinel cattle populations.

Main Methods:

  • Weekly blood sampling from sentinel steers over two years (1986-1987).
  • Virus isolation attempts using embryonated chicken eggs (ECE), baby mice, Aedes albopictus cells, and BHK21 cell monolayers.

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  • Virus identification and characterization of isolated arboviruses.

    • Main Results:

    • Viruses were recovered from 26% of 878 analyzed blood samples.
    • Fourteen arbovirus types were identified, belonging to bluetongue, epizootic haemorrhagic disease (EHD), Palyam, and Simbu groups.
    • Bovine ephemeral fever virus was also isolated.
    • ECE and A. albopictus systems showed high sensitivity for EHD virus isolation, while BHK21 monolayers were optimal for Palyam viruses.

    Conclusions:

    • Diverse arboviruses circulate in cattle populations, necessitating ongoing surveillance.
    • The choice of isolation system significantly impacts the detection efficiency of specific arboviruses.
    • Embryonated chicken eggs (ECE) are effective for bluetongue and Simbu virus isolation.
    • Integrated diagnostic approaches using multiple systems enhance arbovirus detection in veterinary samples.