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Updated: Dec 26, 2025

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
Published on: September 2, 2020
Analysis of phospholipid synthesis in mitochondria
Jorge Montesinos1, Estela Area-Gomez1, Michael Schlame2
1Department of Neurology, Columbia University Medical Center, New York, NY, United States.
Abstract:
Mitochondria and their associated membranes actively participate in biosynthesis, trafficking, and degradation of cellular phospholipids. Two crucial lipid biosynthetic activities of mitochondria include (i) the decarboxylation of phosphatidylserine to phosphatidylethanolamine and (ii) the de novo synthesis of cardiolipin. Here we describe protocols to measure these two activities, applying isotope-labeled or exogenous substrates in combination with thin-layer chromatography or mass spectrometry.
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