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Effect of Yupingfeng granules on the skin barrier in atopic dermatitis mice models
Zhanxue Sun1, Yuhong Kong1, Nan Li1
1Department of Dermatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing100078, China.
Objective:
To investigate effect of Yupingfeng granules, prepared with Chinese Medicines, on the wound healing and on the expression of aquaporin 3 (AQP3) and the skin barrier in the animal models of atopic dermatitis (AD).
Methods:
Acute skin lesions of AD models were prepared using 2,4-dinitrochlorobenzo (DNCB) in mice and animals were treated with either Yupingfeng granules or placebo for two weeks. Skin wound healing outcome was assessed by measuring skin thickness, weight (quality) of the skin, and trans-epidermal water loss (TEWL). Expression of AQP3 mRNA and protein was assessed by reverse transcriotion polymerase chain reaction (RT-PCR) and immunoblotting, respectively.
Results:
Yupingfeng granule treatment resulted in significant acceleration of wound healing with 63.64% efficiency, which was significantly higher than that of placebo granule treatment (31.82%, P < 0.01 by Wilcoxon Rank-sum test). Skin thickness, weight of the wounded skin, and TEWL were significantly higher in the AD models compared to that of normal animals. Treatment with Yupingfeng granules resulted in significant decrease in skin thickness [(937 ± 31) vs (360 ± 21) urn, P < 0.01], weight of the wounded skin [(42 ± 4) vs (24 ± 5) mg, P < 0.01], and TEWL [(30 ± 4) vs (13 ± 4) g•h-1•m-2, P < 0.01]. Yupingfeng granules also significantly down-regulated mRNA and protein expression of AQP3 in the animal models.
Conclusion:
Our findings suggested that Yupingfeng granules could be used in AD treatment.
Insights
Yupingfeng granules significantly accelerated wound healing in atopic dermatitis models by improving skin barrier function and reducing aquaporin 3 (AQP3) expression. This traditional Chinese medicine shows promise for treating atopic dermatitis.
Area of Science:
- Dermatology
- Pharmacology
- Traditional Chinese Medicine
Background:
- Atopic dermatitis (AD) is a chronic inflammatory skin condition characterized by impaired skin barrier function.
- Aquaporin 3 (AQP3) plays a crucial role in skin hydration and barrier integrity.
- Traditional Chinese Medicine (TCM) formulations are explored for their therapeutic potential in AD.
Purpose of the Study:
- To evaluate the efficacy of Yupingfeng granules in promoting wound healing in animal models of atopic dermatitis.
- To investigate the impact of Yupingfeng granules on the expression of aquaporin 3 (AQP3) and skin barrier parameters in AD models.
Main Methods:
- Atopic dermatitis (AD) was induced in mice using 2,4-dinitrochlorobenzo (DNCB).
- Animals were treated with either Yupingfeng granules or placebo for two weeks.
- Wound healing was assessed by measuring skin thickness, weight, and trans-epidermal water loss (TEWL).
- AQP3 mRNA and protein expression were analyzed using RT-PCR and immunoblotting.
Main Results:
- Yupingfeng granules significantly accelerated wound healing (63.64% efficiency) compared to placebo (31.82%, P < 0.01).
- Treatment reduced significantly elevated skin thickness, wounded skin weight, and TEWL in AD models.
- Yupingfeng granules significantly down-regulated AQP3 mRNA and protein expression.
Conclusions:
- Yupingfeng granules demonstrate significant therapeutic effects on wound healing and skin barrier restoration in atopic dermatitis models.
- The findings suggest that Yupingfeng granules may be a viable treatment option for atopic dermatitis.
