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Updated: Dec 26, 2025

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality
Published on: June 2, 2023
Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc
Benjamin J Bowen1, Adam R McGarrity, Jenn-Yeu A Szeto
1School of Biosciences, Cardiff University, Cardiff, UK. jonesdd@cardiff.ac.uk.
None:
Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.
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