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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

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Published on: March 24, 2023

DNA Framework-Based Topological Cell Sorters.

Fangfei Yin^1,2, Min Li³, Xiuhai Mao³

¹Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China.

Angewandte Chemie (International Ed. in English)

|March 19, 2020

Summary

This summary is machine-generated.

Researchers engineered tetrahedral DNA frameworks (TDFs) to create topologically controlled ligands. This novel approach significantly enhances molecular binding strength by inducing receptor clustering, enabling precise control over cell interactions.

Keywords:

DNA nanostructures cell sorting framework nucleic acid topological engineering

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Area of Science:

Biochemistry and Molecular Biology
Nanotechnology
Cell Biology

Background:

Molecular recognition in cellular processes relies on specific ligand-receptor interactions on cell membranes.
Existing ligand systems offer limited control over topological organization and its impact on binding strength.
Understanding ligand-receptor topological organization is crucial for manipulating cellular interactions.

Purpose of the Study:

To develop a novel system for topologically controlled ligands.
To investigate the effect of ligand topology on molecular recognition and binding strength.
To demonstrate the application of topologically engineered ligands in cell patterning and sorting.

Main Methods:

Fabrication of a family of tetrahedral DNA frameworks (TDFs) to arrange multiple ligands stoichiometrically and topologically.
Utilizing TDFs to induce receptor clustering through topological control of ligand presentation.
Quantifying the change in binding strength due to topological ligand organization.

Main Results:

Successful generation of topologically controlled ligands using TDFs.
Demonstrated significant enhancement (approximately 10-fold) in binding strength due to induced receptor clustering.
Showcased the utility of TDFs for effective cell patterning and cell sorting applications.

Conclusions:

Topological control of ligand arrangement via TDFs fundamentally alters molecular recognition.
This approach offers a versatile platform for designing advanced topological ligands.
The developed TDF system provides precise control over binding strength and cellular interactions.