Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phenotype-Specific Recalibration of MAVE Data Enables Repurposing of <i>BAP1</i> Functional Assays for Küry-Isidor Syndrome.

medRxiv : the preprint server for health sciences·2026

Same author

Retinoic acid regulates foveal development in the human retina.

Cell reports·2026

Same author

Modular genetic architecture underlies human hand and foot evolution.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Genome-wide detection and clinical prioritization of tandem repeat outliers using long-read sequencing.

medRxiv : the preprint server for health sciences·2026

Same author

Comprehensive mapping of Neurofibromin (NF1) expression in developing mouse brain.

bioRxiv : the preprint server for biology·2026

Same author

Spatiotemporal histogenesis of the developing human cerebellum reveals dynamic layering of Bergmann glia.

Proceedings of the National Academy of Sciences of the United States of America·2026

Search research articles

Related Experiment Video

Updated: Dec 26, 2025

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Laser Capture Micro-dissection (LCM) of Neonatal Mouse Forebrain for RNA Isolation.

Achira Roy¹, Mei Deng², Kimberly A Aldinger^1,2

¹Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, United States.

|March 20, 2020

Summary

This summary is machine-generated.

Precise isolation of specific cells from mouse forebrains using laser capture microdissection (LCM) preserves tissue integrity for genomic analysis. This improved protocol simplifies cell collection for studying brain development and disorders.

Keywords:

Forebrain Hippocampus Laser capture microdissection Microscopy Mouse Neural progenitors RNA isolation RNA sequencing

More Related Videos

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

Related Experiment Videos

Last Updated: Dec 26, 2025

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

Area of Science:

Neuroscience
Molecular Biology
Genomics

Background:

Analyzing gene expression in the forebrain requires isolating specific cell types from heterogeneous tissue.
Traditional methods lose spatial and molecular resolution due to cell pooling.
Understanding forebrain development is key to addressing brain disorders.

Purpose of the Study:

To present a simplified and user-friendly protocol for precise cell isolation from fresh-frozen mouse forebrains.
To enable high-throughput genomic and transcriptomic analyses with preserved in vivo tissue integrity.
To facilitate molecular studies of forebrain development in normal and diseased states.

Main Methods:

Cryo-sectioning of fresh-frozen mouse forebrains (genetically modified and therapeutically treated).
Laser capture microdissection (LCM) under RNase-free conditions to isolate specific cell types or layers.
Adaptation of the protocol for various brain regions and model organisms, including human tissue.

Main Results:

Achieved precise and reproducible isolation of homogeneous cell populations.
Maintained in vivo tissue integrity, spatial, molecular, and cellular resolution.
Developed a simplified protocol with break-points for enhanced usability without compromising quality.

Conclusions:

The refined LCM protocol provides a powerful tool for detailed molecular analysis of specific neural cell populations.
This method supports quantitative studies in neuroscience, aiding the understanding of brain development and disorders.
The protocol's adaptability makes it broadly applicable across different tissues and research models.