Related Experiment Video
Updated: Dec 25, 2025
09:23
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
Published on: April 2, 2020
6.8K
lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition
Jun-Qi Liu1, Ming Deng2, Nan-Nan Xue1
1Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China.
Molecular Therapy. Nucleic Acids
|March 21, 2020
Summary
Long non-coding RNA KLF3-AS1 inhibits esophageal squamous cell carcinoma (ESCC) progression by targeting miR-185-5p. Upregulating KLF3-AS1 reduces tumor growth and spread, offering a potential new treatment for ESCC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Esophageal squamous cell carcinoma (ESCC) is a prevalent malignancy globally.
- Long non-coding RNAs (lncRNAs) play significant roles in cancer development, but their specific functions in ESCC are not fully understood.
Purpose of the Study:
- To investigate the role of lncRNA Krüppel-like factor 3 antisense RNA 1 (KLF3-AS1) in ESCC.
- To elucidate the regulatory mechanism of KLF3-AS1 involving miR-185-5p and KLF3 in ESCC.
Main Methods:
- Preparation and characterization of ESCC cell spheres with stem cell-like properties.
- Assessment of KLF3-AS1 expression in ESCC.
- Analysis of the interaction between KLF3-AS1, miR-185-5p, and KLF3.
- Evaluation of KLF3-AS1's effects on ESCC cell behaviors in vitro and tumor growth in vivo.
Main Results:
- KLF3-AS1 was found to be downregulated in ESCC.
- KLF3-AS1 upregulates KLF3 expression by binding to miR-185-5p, leading to decreased SOX2 and Oct4 expression.
- Upregulation of KLF3-AS1 inhibited ESCC cell colony formation, invasion, migration, and tumor volume, while promoting apoptosis.
Conclusions:
- lncRNA KLF3-AS1 acts as a tumor suppressor in ESCC.
- KLF3-AS1 inhibits ESCC progression by regulating the miR-185-5p/KLF3 axis.
- KLF3-AS1 represents a potential therapeutic target for ESCC treatment.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
9.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
01:22MicroRNAs
23.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
01:22MicroRNAs
3.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
01:35Cancer Cell Migration through Invadopodia
3.1K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.1K