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Updated: Dec 25, 2025

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Published on: April 18, 2019
Evaluation of the susceptibility test of polymyxin B using the commercial test Policimbac®
Tanise Vendruscolo Dalmolin1,2, Maiara Dos Santos Carneiro1,2, Luíza Peres de Castro1
1Laboratório de Pesquisa em Resistência Bacteriana-LABRESIS, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
Abstract:
Broth microdilution (BMD), the reference method to determine bacterial susceptibility to polymyxins, is a laborious and time-consuming technique. Policimbac® is a commercial test panel which uses lyophilized polymyxin B to determine the minimum inhibitory concentration for Gram-negative isolates. This study evaluated the performance of Policimbac® in comparison with BMD for 110 isolates. Although the Policimbac® presented a very low essential agreement, the categorical agreement with BMD was optimal. Policimbac® is an alternative approach to BMD for evaluating the susceptibility to polymyxin B.
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