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Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.5K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: Dec 25, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

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Research Techniques Made Simple: CRISPR Genetic Screens.

Auke B C Otten1, Bryan K Sun1

  • 1Department of Dermatology, University of California San Diego, La Jolla, California, USA.

The Journal of Investigative Dermatology
|March 24, 2020
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas systems enable high-throughput genetic screens for gene function discovery. This review covers designing, executing, and analyzing CRISPR screens, including applications in dermatology.

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • CRISPR/Cas systems are bacterial defense mechanisms adapted for genome editing.
  • CRISPR/Cas enables targeted gene perturbation, particularly for generating genetic loss-of-function.
  • High-throughput CRISPR screens combined with deep sequencing accelerate the identification of genes involved in specific phenotypes.

Purpose of the Study:

  • To provide a comprehensive overview of CRISPR screening methodologies.
  • To discuss key considerations in the design, execution, and analysis of CRISPR screens.
  • To highlight the versatility of CRISPR/Cas for various genetic modifications and its application in investigative dermatology.

Main Methods:

  • Focus on CRISPR knockout screens for loss-of-function studies.
  • Review adaptations of CRISPR/Cas for diverse genetic perturbations.
  • Integration with deep sequencing for high-throughput analysis.

Main Results:

  • CRISPR screens offer a powerful tool for large-scale genetic analysis.
  • The system's versatility extends beyond knockout to other genetic modifications.
  • Successful application examples in investigative dermatology are presented.

Conclusions:

  • CRISPR screening is a versatile and powerful approach for functional genomics.
  • Methodological considerations are crucial for successful screen design and execution.
  • CRISPR screens hold significant potential for advancing research in fields like dermatology.