Coupled graphene oxide with hybrid metallic nanoparticles as potential electrochemical biosensors for precise...

Ascorbic acid (AA) as an essential biological molecule for proper performance of body can act as a biological metric for precise detection of various kinds of disease through measuring the level of oxidative stress; thus its precise/dividable detection is an urgent requirement for development of advanced biosensors. To address this requirement, we decorated well-exfoliated graphene oxide (GO) with Ag and hybrid Ag-Fe₃O₄ metallic nanoparticles toward precise, real-time and repeatable detection of AA within the blood plasma samples via electrochemical approaches that led to the development of a retrievable biosensor. Outcome of performed evaluations showed that modification of glassy carbon electrode (GCE) with selected additives significantly improved its sensitivity/selectivity. In this matter, the modified GCE with GO-Ag-Fe₃O₄ showed limit of detection and sensitivity of 74 nM and 1146.8 μA mM^-1 cm^-2, respectively, within the concentration range of 0.2-60 μM. Additionally, the modified electrode kept 91.23% of its total performance after 15 days of performance and detected the oxidation peak of AA even with present of 50 fold of annoying contents which highlighting its superior stability/selectivity. More importantly, the developed electrode showed recovery range between 96.0 and 104.4% within the human blood plasma samples that confirmed the ideal capability of developed platform for accurate detection of AA within biological fluids.