Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

1.3K
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
1.3K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

618
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
618
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.6K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.6K
The Equilibrium Constant03:10

The Equilibrium Constant

55.2K
Consider the oxidation of sulfur dioxide:
55.2K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

911
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
911
¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

1.2K
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revision of the circumglobal genus Barathronus (Ophidiiformes, Bythitidae) with a new species from the eastern North Atlantic Ocean.

Zootaxa·2019
Same author

Aphyonid-clade species of Australia (Teleostei, Bythitidae) with four species new to Australian waters and a new species of Barathronus.

Zootaxa·2019
Same author

Impact of leaks in sulfur hexafluoride and nitrogen multiple-breath washout systems.

ERJ open research·2018
Same author

Difference between SF<sub>6</sub> and N<sub>2</sub> multiple breath washout kinetics is due to N<sub>2</sub> back diffusion and error in N<sub>2</sub> offset.

Journal of applied physiology (Bethesda, Md. : 1985)·2018
Same author

Review of the steatiticus-species group of the cuskeel genus Neobythites (Ophidiidae) from the Indo-Pacific, with description of two new species.

Zootaxa·2018
Same author

Nitrogen back-diffusion during multiple-breath washout with 100% oxygen.

The European respiratory journal·2017

Related Experiment Video

Updated: Dec 25, 2025

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.0K

Nitrogen offset in N2 multiple washout method.

Jørgen G Nielsen1

  • 1Borreby Holding ApS, Glamsbjerg, Denmark.

ERJ Open Research
|March 24, 2020
PubMed
Summary

An offset in the nitrogen signal significantly impacts Leaf Component Index (LCI) measurements. Proper calibration is crucial for accurate LCI data in plant science research.

Area of Science:

  • Plant physiology
  • Remote sensing
  • Agricultural science

Background:

  • Leaf Component Index (LCI) is a key metric for assessing plant health and productivity.
  • Accurate LCI measurement is vital for agricultural monitoring and research.
  • Nitrogen signal calibration is a critical step in LCI data acquisition.

Purpose of the Study:

  • To investigate the impact of nitrogen signal offset on LCI measurements.
  • To determine the sensitivity of LCI to nitrogen signal variations.
  • To provide recommendations for improving LCI measurement accuracy.

Main Methods:

  • Utilized controlled experimental setups to simulate nitrogen signal offsets.
  • Collected LCI data under varying offset conditions.

More Related Videos

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.5K
Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

8.6K

Related Experiment Videos

Last Updated: Dec 25, 2025

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.0K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.5K
Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

8.6K
  • Analyzed the correlation between nitrogen signal offset and LCI values.

    • Main Results:

    • A significant offset in the nitrogen signal was found to substantially affect LCI readings.
    • The magnitude of the LCI deviation was directly proportional to the nitrogen signal offset.
    • Uncorrected offsets lead to unreliable LCI data.

    Conclusions:

    • Nitrogen signal calibration is essential for accurate LCI determination.
    • Implementing rigorous calibration protocols can enhance the reliability of LCI-based assessments.
    • Addressing signal offsets is critical for advancing plant phenotyping and precision agriculture.