Powering Artificial Enzymatic Cascades with Electrical Energy

Area of Science:

• Biocatalysis
• Synthetic Chemistry
• Electrochemistry

Background:

• Enzymatic cascades offer efficient synthesis routes but often require specific redox mediators.
• Electrolysis can provide a sustainable source of electrons for biocatalytic reactions.
• Continuous flow reactors enhance reaction efficiency and scalability.

Purpose of the Study:

• To develop a scalable platform using electrolysis for in vitro synthetic enzymatic cascades.
• To utilize hydrogen and oxygen produced via electrolysis as electron mediators for biocatalysts.
• To demonstrate the synthesis of methylated N-heterocycles and regioselective labeling with stable isotopes.

Main Methods:

• Development of a continuous flow reactor system.
• Integration of electrolysis for hydrogen and oxygen production.
• Use of a gas-permeable membrane for separating electrolyte from biocatalysts.
• Application of H2 and O2 as electron mediators for oxidoreductases.

Main Results:

• Achieved up to 99% product formation in the synthesis of methylated N-heterocycles from diamines.
• Demonstrated excellent regioselective labeling with stable isotopes.
• Successfully employed a broad panel of oxidoreductases.

Conclusions:

• The developed platform offers a scalable and efficient method for enzymatic synthesis using electrical energy.
• Electrolysis-driven biocatalysis provides a sustainable alternative for fine chemical production.
• The platform is versatile and applicable to various oxidoreductases.