Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.8K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An end-to-end hybrid deep-learning approach for single-shot wavefront sensing and correction.

Nature communications·2026
Same author

<i>In vivo</i> fundus imaging and computational refocusing with a diffuser-based fundus camera.

Biophotonics discovery·2026
Same author

Dual-channel event microscopy for ultrafast biological imaging.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The neurovascular impulse response function differentially reflects intrinsic neuromodulation across cortical regions.

Nature neuroscience·2026
Same author

Optical sectioning in wide-field two-photon microscopy using temporal focusing and random illumination.

Optics letters·2026
Same author

QuATON: quantization aware training of optical neurons.

Optics express·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Dec 25, 2025

Scattering And Absorption of Light in Planetary Regoliths
11:34

Scattering And Absorption of Light in Planetary Regoliths

Published on: July 1, 2019

10.8K

Inverse scattering for reflection intensity phase microscopy.

Alex Matlock1, Anne Sentenac2, Patrick C Chaumet2

  • 1Department of Electrical and Computer Engineering, Boston University, Boston, MA 02215, USA.

Biomedical Optics Express
|March 25, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a new reflection phase microscopy method using only intensity measurements. It enables label-free, high-resolution imaging of biological samples, enhancing contrast for weakly scattering specimens.

More Related Videos

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Published on: September 8, 2016

10.7K
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

16.1K

Related Experiment Videos

Last Updated: Dec 25, 2025

Scattering And Absorption of Light in Planetary Regoliths
11:34

Scattering And Absorption of Light in Planetary Regoliths

Published on: July 1, 2019

10.8K
Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Published on: September 8, 2016

10.7K
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

16.1K

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Microscopy

Background:

  • Reflection phase microscopy typically relies on interferometric techniques for label-free, high-resolution imaging.
  • Existing methods face challenges in accurately recovering object phase due to combined linear and nonlinear scattering effects.

Purpose of the Study:

  • To investigate reflection phase microscopy using only intensity measurements.
  • To develop and evaluate a linear inverse scattering model for imaging scattering objects.
  • To enhance contrast for thin, weakly scattering biological samples.

Main Methods:

  • Utilizing intensity-only measurements under diverse illumination.
  • Applying the first Born approximation for forward and inverse scattering models.
  • Deriving a linear inverse scattering model based on forward-scattering components.
  • Experimental validation using a modified standard reflection microscope with a programmable light source.

Main Results:

  • Successfully derived and evaluated a linear inverse scattering model.
  • Demonstrated enhanced contrast for thin, weakly scattering samples.
  • Showcased the model's validity range through simulations and experiments.

Conclusions:

  • The developed intensity-based reflection quantitative phase imaging model offers a simplified approach.
  • This method complements traditional transmission techniques for biological sample characterization.
  • The system is promising for easy adoption in biological research settings.