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Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling
Jeremy M Crook1,2,3, Eva Tomaskovic-Crook4,5
1ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, University of Wollongong, Wollongong, NSW, Australia. jcrook@uow.edu.au.
Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2020
Summary
3D bioprinting using human induced pluripotent stem cells (iPSCs) creates robust, self-replicating cell constructs. These constructs can be differentiated into various human tissues for research and clinical applications.
Area of Science:
- Regenerative Medicine
- Biotechnology
- Stem Cell Biology
Background:
- Human pluripotent stem cells (PSCs) are key to developing 3D tissue constructs.
- Bioprinting offers a method for precise cell placement in 3D scaffolds.
- Existing methods require refinement for clinical applicability.
Purpose of the Study:
- To detail a method for 3D bioprinting human induced pluripotent stem cells (iPSCs).
- To demonstrate the viability and potential of bioprinted iPSCs within a functional construct.
- To highlight the application of this technique for diverse tissue engineering.
Main Methods:
- Utilizing a previously published method for 3D printing human iPSCs.
- Encapsulating iPSCs within a clinically amenable bioink.
- Cross-linking the bioink to form a stable 3D construct.
Main Results:
- Printed iPSCs maintained self-replication and multilineage potential in situ.
- The resulting 3D constructs were robust and stable.
- Constructs demonstrated amenability to various differentiation protocols.
Conclusions:
- 3D bioprinting of iPSCs is feasible using the described method and bioink.
- This technique supports the development of diverse, functional human tissues.
- The approach holds potential for both research and clinical product development.
