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Fibroblast growth factor receptor 1 gene amplification and protein expression in human lung cancer

Omar Elakad1, Anna-Maria Lois1, Katja Schmitz1

  • 1Institute of Pathology, University Medical Center, Göttingen, Germany.

Cancer Medicine
|March 25, 2020
PubMed
Abstract

Insights

Fibroblast growth factor receptor 1 (FGFR1) amplification and expression do not impact lung cancer patient survival. Current patient selection for FGFR1 inhibitors may need reconsideration based on these findings.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Targeting fibroblast growth factor receptor 1 (FGFR1) shows promise for squamous cell lung cancer (SQCLC).
  • Current treatment decisions rely on FGFR1 gene amplification, but durable responses are limited.
  • Previous research on FGFR1's prognostic impact in lung cancer yields conflicting results.

Purpose of the Study:

  • To investigate the prevalence and correlation of FGFR1 gene amplification and protein expression in lung cancer.
  • To determine the impact of FGFR1 amplification and expression on overall survival.
  • To evaluate the relationship between FGFR1 amplification, expression, and clinicopathological characteristics.

Main Methods:

  • Analyzed FGFR1 gene amplification using fluorescence in situ hybridization in 208 SQCLC and 45 small cell lung cancers (SCLC).
  • Assessed FGFR1 protein expression via immunohistochemistry in SQCLC, SCLC, and 121 pulmonary adenocarcinomas (ACs).
  • Correlated amplification and expression with clinicopathological features and overall survival.

Main Results:

  • FGFR1 amplification occurred in 23% of SQCLC and 8% of SCLC, correlating with male patients but not survival.
  • Strong FGFR1 expression was observed in 9% of SQCLC, 35% of ACs, and 4% of SCLCs, correlating with females and absence of lymph node metastasis in SQCLC, but not survival.
  • No significant correlation was found between FGFR1 gene amplification and protein expression.

Conclusions:

  • FGFR1 gene amplification does not correlate with its protein expression in lung cancer.
  • Neither FGFR1 amplification nor protein expression appears to influence patient prognosis.
  • Patient selection for FGFR1 inhibitors warrants re-evaluation.
Keywords:
FGFR1CRISPR/Cas9fluorescence in situ hybridizationimmunohistochemistrylung cancer

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