Temperature-dependent effects on CO₂ water bath therapy induced changes in blood flow and vascularity in hind limb...

To test if magnitudes of the beneficial actions of CO₂ water bath therapy on blood flow and vascular density are dependent upon temperature, ischemia in the hind limb of rats was induced by occluding the left femoral artery for 2 weeks and the animals were exposed to water bath therapy with or without CO₂ at 34 or 41 °C for 4 weeks (20 min treatment each day for 5 days/week). CO₂ water bath therapy at 34 °C increased peak, minimal, and mean blood flow by 190%-600% in the ischemic limb. On the other hand, CO₂ water bath treatment at 41 °C increased these parameters of blood flow by 37%, 55%, and 41%, respectively, in the ischemic limb. The small blood vessel count, an index of vascular density, in the ischemic limb was increased by CO₂ water bath therapy at 34 and 41 °C by 32% and 122%, respectively. No changes in the ischemic animals by CO₂ water bath therapy at 34 or 41 °C were observed in the heart rate, R-R interval, and plasma lipid or glucose levels. These data indicate that the beneficial effect of CO₂ water bath therapy at 34 °C on blood flow in the ischemic muscle is greater whereas that on vascular density is smaller than changes in these parameters at 41 °C.