Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

503
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
503

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyanidin-3-glucoside mitigates zearalenone-enhanced hepatic lipotoxicity via inhibition of PXR activation.

Food research international (Ottawa, Ont.)·2026
Same author

[Effects of extensively hydrolyzed protein formula on sensitization and gut microbiota in mice].

Wei sheng yan jiu = Journal of hygiene research·2026
Same author

Portulaca Oleracea polysaccharides attenuate zearalenone-induced macrophage activation and Leydig cell dysfunction in testis: A single-cell and multi-omics study.

Food research international (Ottawa, Ont.)·2026
Same author

Lactoferrin Deficiency During Lactation Causes Adult Obesity-Related Metabolic Disease Through Persistent Adipose Dysfunction Driven by Impaired Adipocyte Development.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Comparative Microbiota and Metabolite Profiles of Undried and Dried Typica Luwak (Civet) Coffee Beans.

Foods (Basel, Switzerland)·2026
Same author

Broccoli-Derived Exosome-like Nanoparticles Alleviates Metabolic Dysfunction-Associated Steatotic Liver Disease Through Modulating the Gut-Liver Axis.

Nutrients·2026

Related Experiment Video

Updated: Dec 25, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.6K

Multiplex pyrosequencing quantitative detection combined with universal primer-multiplex-PCR for genetically modified

Ying Shang1, Yuancong Xu2, Kunlun Huang3

  • 1Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Faulty of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Food Chemistry
|March 26, 2020
PubMed
Summary

A novel quantitative detection method using multiplex pyrosequencing and universal primer-multiplex-PCR (UP-M-PCR) accurately quantifies genetically modified (GM) lines. This technique offers high sensitivity for GMO detection without fluorescent labeling.

Keywords:
Base sequence degeneracyGMOMatrix equationMultiple pyrosequencingQuantitative detectionUniversal primer-multiplex-PCR

More Related Videos

Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

27.9K
Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
07:42

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes

Published on: January 10, 2022

4.6K

Related Experiment Videos

Last Updated: Dec 25, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.6K
Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

27.9K
Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
07:42

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes

Published on: January 10, 2022

4.6K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Accurate quantification of genetically modified (GM) organisms is crucial for regulatory compliance and food labeling.
  • Existing detection methods may have limitations in sensitivity, multiplexing capability, or require fluorescent labeling.

Purpose of the Study:

  • To establish a novel, sensitive, and reliable quantitative detection technique for multiple genetically modified (GM) lines.
  • To develop a self-compiled software for analyzing pyrosequencing data and converting it into mathematical relations for quantification.

Main Methods:

  • A multiplex pyrosequencing quantitative detection technique was combined with universal primer-multiplex-PCR (UP-M-PCR).
  • Self-compiled software was developed to analyze pyrosequencing results, including DNA sequence degeneration and base composition.
  • Five calculation models were created to adjust values below zero or the detection limit.

Main Results:

  • The method accurately quantified samples containing five GM lines mixed in random ratios, with results closely matching actual values.
  • The detection sensitivity achieved was as low as 1.47% for a single component.
  • The technique demonstrated reliability and satisfied most labeling policies.

Conclusions:

  • The developed multiplex pyrosequencing and UP-M-PCR method is a reliable tool for quantitative detection of GM lines.
  • The absence of fluorescent labeling removes limitations on the number of targets, enhancing versatility.
  • This technique offers high sensitivity and accuracy, suitable for various labeling policies.