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Related Experiment Videos

Isolation of basic drugs from plasma using solid-phase extraction with a cyanopropyl-bonded phase.

G Musch1, D L Massart

  • 1Vrije Universiteit Brussel, Farmaceutisch Instituut, Brussels, Belgium.

Journal of Chromatography
|November 18, 1988
PubMed
Summary

A novel cyano (CN) sorbent effectively isolates basic drugs from plasma, showing minimal matrix interference. This method achieves high recoveries for various therapeutic drugs, enhancing analytical accuracy.

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Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Biochemistry

Background:

  • Accurate quantification of basic drugs in plasma is crucial for therapeutic drug monitoring.
  • Existing methods for isolating basic compounds from biological matrices can be complex and time-consuming.

Purpose of the Study:

  • To evaluate the efficacy of a cyano (CN) sorbent for the isolation of basic compounds from plasma.
  • To determine optimal conditions for adsorption, washing, and elution of basic drugs.
  • To assess the method's applicability for quantifying therapeutic drug levels in plasma.

Main Methods:

  • Investigated adsorption of 30 basic drugs from water and plasma using a CN sorbent.
  • Optimized elution solvents (methanol-phosphate buffer and methanol with propylamine) and washing steps (water).

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  • Applied the developed method to determine eight drugs in plasma at therapeutic concentrations.

    • Main Results:

    • Compounds with a carbon chain length >= 11 were fully retained on the CN sorbent.
    • Matrix effects from plasma were minimal, simplifying sample preparation.
    • High absolute recoveries (82-99%) were achieved for eight basic drugs at therapeutic levels.

    Conclusions:

    • The CN sorbent provides an efficient and reliable method for isolating basic drugs from plasma.
    • The method is suitable for determining therapeutic drug levels with high accuracy and precision.
    • This approach simplifies sample preparation and enhances analytical workflows in clinical and forensic toxicology.