[Differentially expressed long non-coding RNAs in spermatozoa with different fertilizing abilities].

Area of Science:

• Reproductive Biology
• Molecular Genetics
• Bioinformatics

Background:

• Fertilization is a complex process influenced by numerous molecular factors.
• Long non-coding RNAs (lncRNAs) are emerging as critical regulators in various biological functions, including reproduction.
• Understanding the role of lncRNAs in sperm function is crucial for assessing male fertility.

Purpose of the Study:

• To identify conception capacity-related long non-coding RNAs (lncRNAs) in human semen.
• To investigate the potential roles of these lncRNAs in the process of fertilization.
• To explore the utility of lncRNAs as biomarkers for sperm quality.

Main Methods:

• Semen samples from individuals with high and low fertilizing ability were collected.
• RNA sequencing was performed to identify and quantify lncRNAs.
• Bioinformatic analyses, including differential expression screening, functional enrichment (GO, pathway), and interaction analysis, were employed.

Main Results:

• A total of 147,1615 lncRNAs were identified, with 4,052 showing differential expression between groups.
• Differentially expressed lncRNAs were involved in metabolic processes, biological regulation, and signaling pathways.
• Specific lncRNAs were predicted to be associated with sperm motility and acrosomal reaction, key events in fertilization.

Conclusions:

• Differentially expressed lncRNAs play a significant role in human fertilization.
• These lncRNAs hold promise as novel biomarkers for assessing sperm quality and male fertility.
• Further research into lncRNA functions can advance reproductive medicine.