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Development of a highly sensitive ELISA for determination of darunavir in plasma samples using a polyclonal antibody
Abdulrahman A Almehizia1, Rashed N Herqash1,2, Ibrahim A Darwish1
1Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Abstract:
Aim: To support pharmacokinetic studies and therapeutic monitoring of darunavir (DRV), a highly sensitive ELISA was developed for the determination of DRV in plasma samples at picogram levels. Results: The assay LOD and LOQ were 15 and 30 pg ml-1, respectively. The working range of the assay was 20-2000 pg ml-1. Analytical recoveries of DRV from spiked plasma were in the ranges of 98.4-113.0 and 86.0-99.1% for intra-assay and inter-assay runs, respectively. The precision of the assay was satisfactory. Conclusion: The ELISA is characterized by high throughput and it is expected to significantly contribute to routine analysis of DRV in its pharmacokinetic studies and therapeutic monitoring.
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