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Vascularized cardiac tissue construction with orientation by layer-by-layer method and 3D printer.

Yoshinari Tsukamoto1, Takami Akagi1, Mitsuru Akashi2

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Summary

Researchers created 3D cardiac tissue with aligned cells and blood vessels using 3D printing and layer-by-layer techniques. This engineered heart tissue shows improved contractile function, advancing regenerative medicine and drug testing.

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Area of Science:

  • Biomaterials Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Developing functional 3D cardiac tissue is crucial for regenerative medicine and pharmaceutical applications.
  • Existing methods often lack control over cellular orientation and vascularization, limiting tissue function.

Purpose of the Study:

  • To fabricate native organ-like 3D cardiac tissue with an oriented structure and vascular network.
  • To evaluate the contractile function of orientation-controlled 3D cardiac tissue.
  • To assess the potential of this engineered tissue for regenerative medicine and drug discovery.

Main Methods:

  • Utilized a layer-by-layer (LbL) technique combined with cell accumulation and 3D printing.
  • Employed hydroxybutyl chitosan (HBC), a thermoresponsive polymer, for 3D shaping.
  • Incorporated human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM), normal human cardiac fibroblasts (NHCF), and human cardiac microvascular endothelial cells (HMVEC).

Main Results:

  • Successfully fabricated orientation-controlled 3D cardiac tissue with aligned hiPSC-CM and NHCF.
  • Demonstrated enhanced contractile function in orientation-controlled tissue compared to uncontrolled tissue.
  • Established a vascular network within the 3D cardiac tissue through co-culture with HMVEC.

Conclusions:

  • The developed 3D cardiac tissue exhibits an oriented structure and functional vascular network.
  • This engineered tissue shows superior contractile properties, making it promising for cardiac repair.
  • The fabricated 3D cardiac tissue serves as a valuable platform for regenerative medicine and pharmaceutical research.