The major fraction of deoxyribonuclease activity from human urinary proteins. Purification and properties

The major fraction of deoxyribonuclease activity from human urinary protein was purified 40-fold in about 14% yield. The enzyme shows an isoelectric point at pH 4.2 and has a molecular weight of 33,600+/-3,000. Optimum activity was shown at pH 6.8 in the presence of 12.5 mmol/l Mg2+ plus 1 mmol/l Ca2+. The enzymic reaction is inhibited by high ionic strength (greater than 300 mmol/l Na+). The purified enzyme readily hydrolyzes native DNA to oligodeoxyribonucleotides with an average chain length of 5.3+/-0.2 after exhaustive digestion. Therefore, this endonuclease may be designated as neutral deoxyribonuclease (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.5).