Neutrophil-derived exosome from systemic sclerosis inhibits the proliferation and migration of endothelial cells
Related Experiment Video
Updated: Dec 25, 2025
Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice
Published on: April 10, 2019
Liya Li1, Xiaoxia Zuo1, Yizhi Xiao1
1Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China; The Institute of Rheumatology and Immunology, Central South University, Changsha, China.
Objectives:
Systemic sclerosis (SSc) is an autoimmune disease characterized by vasculopathy, inflammation, and extensive fibrosis in multiple organs. Exosomes (EXOs) are cell-derived vesicles contained various DNAs, RNAs and proteins, and play important roles in various diseases. Here, we aimed to investigate the roles of SSc EXOs in angiogenesis related mechanisms.
Methods:
EXOs were isolated from plasma, cultured peripheral blood mononuclear cells (PBMCs)/neutrophil supernatants, and identified by transmission electron microscopy. The expression of S100A8/A9 was measured by real-time PCR and ELISA. Proliferation, migration and scratch assays in human dermal microvascular endothelial cells (HDMECs) were used to study the EXOs influence.
Results:
Plasma and neutrophil EXOs from SSc patients can suppress the proliferation and migration of HDMECs. High levels of S100A8/A9 were found in SSc EXOs which derived from plasma, PBMCs and neutrophils. The expression of S100A8/A9 in neutrophil EXOs was higher than that in PBMC EXOs in SSc patients. The proliferation and migration of HDMECs were possibly inhibited by S100A8/A9 of neutrophil EXOs.
Conclusions:
Neutrophil EXOs from SSc patients inhibits the proliferation and migration of HDMECs, S100A8/A9 might play an important role in this process.
