Highly sensitive vertical flow based point-of-care immunokit for rapid and early detection of human CRP as a...

Early detection and treatment of cardiovascular disease by identifying markers is important to improve the health of our citizens and simultaneously reduce the cost of health care. Therefore, there is an urgent need for early detection and treatment of asymptomatic cardiovascular disease. Herein, we have developed highly sensitive vertical flow immunokit (VFIK) for the detection of C-reactive protein (CRP), comprising an antibody/citrate conjugated gold nanoparticle in a fixed orientation surface. Our CRP test kit has been optimized with various parameters such as buffer composition, the quantity of captured antibody and sensitivity of the assay. Prominently, our developed CRP test kit is highly sensitive and rapid point of care test (POCT) which is capable to detect CRP with minimal volume (50-60 μl) and time (1-2 min) with diminutive detection up to 10 ng/ml. The reliability of our kit was evaluated and validated with spiked and clinical whole blood samples. The results demonstrated that our developed vertical flow-based point-of-care Immunokit may serve fruitful and semi-quantitative routine diagnostic for the early detection of cardiac disease.