Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Real-world outcomes of [<sup>177</sup>Lu]Lu-PSMA-I&T in [<sup>18</sup>F]FDG-positive metastatic castration-resistant prostate cancer: factors related to response and survival.

European journal of nuclear medicine and molecular imaging·2026

Same author

Early cAMP signaling orchestrates single-cell synchronicity throughout Dictyostelium development.

Communications biology·2026

Same author

Mutual suppression between mutations in the Dictyostelium Greenbeard pathway restores wild-type development.

BMC genomics·2025

Same author

Author Correction: Global influence of soil texture on ecosystem water limitation.

Nature·2025

Same author

Digital Twins for 3D Confocal Microscopy: Near-Field, Far-Field, and Comparison with Experiments.

Sensors (Basel, Switzerland)·2025

Same author

Going against the family: Perturbation of a greenbeard pathway leads to falsebeard cheating.

iScience·2024

Search research articles

Related Experiment Video

Updated: Dec 25, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Microsphere-assisted interferometry with high numerical apertures for 3D topography measurements.

Lucie Hüser, Peter Lehmann

|April 1, 2020

Summary

Researchers used microspheres to achieve superresolution imaging beyond the diffraction limit. This study provides deeper insight into the physical mechanisms enabling near-field to far-field information transfer for enhanced optical metrology.

More Related Videos

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Related Experiment Videos

Last Updated: Dec 25, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Area of Science:

Optical Metrology
Nanotechnology
Microscopy

Background:

Improving lateral resolution in optical measuring systems is crucial for expanding optical metrology applications.
Microspheres as near-field supports have demonstrated the possibility of superresolution below Abbe's diffraction limit.

Purpose of the Study:

To provide detailed theoretical and experimental insights into the physical mechanisms of near-field to far-field information transfer.
To investigate the effects of microspheres as near-field supports on phase-evaluating interference microscopes.
To present measurement results of sub-diffraction limit grating structures.

Main Methods:

Experimental investigations using microspheres as near-field supports.
Numerical simulations of optical systems.
Measurements with a high numerical aperture Linnik microscope.
Analysis of phase-evaluating interference microscopy.

Main Results:

Demonstrated superresolution imaging of structures below Abbe's diffraction limit.
Provided detailed understanding of physical mechanisms for near-field to far-field information transfer.
Presented experimental and simulated data on microsphere effects in interference microscopy.

Conclusions:

Microspheres effectively act as near-field supports, enabling superresolution in optical metrology.
Understanding the physical mechanisms is key to advancing optical resolution limits.
The study validates superresolution techniques for sub-diffraction limit feature analysis.