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Immunoprecipitation

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Related Experiment Video

Updated: Dec 25, 2025

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

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Published on: September 12, 2019

High-Efficiency Plasma Separator Based on Immunocapture and Filtration.

Xiaosong Su^1,2,3, Jianzhong Zhang^1,2,3, Dongxu Zhang^1,2,3

¹State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen 361102,China.

|April 2, 2020

Summary

A novel microfluidic device offers a rapid, cost-effective solution for plasma separation at the point-of-care. This immunological capture and filtration method simplifies blood processing for clinical diagnostics.

Keywords:

microfluidics application plasma separation point-of-care testing sample preparation separator whole blood

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Area of Science:

Biomedical Engineering
Clinical Diagnostics
Microfluidics

Background:

Standard plasma separation methods are cumbersome, expensive, and not suitable for point-of-care applications.
Limitations include inconvenience, labor intensity, and high costs, hindering bedside clinical use.

Purpose of the Study:

To develop a new, convenient, cost-effective plasma separation technique and device.
To overcome the limitations of traditional plasma preparation methods for point-of-care use.

Main Methods:

Development of a novel device utilizing immunological capture and filtration for plasma separation.
Antibody-coated separation matrix immobilizes Red Blood Cells (RBCs).
Commercially available plasma purification membranes remove residual cells.

Main Results:

The device processes 400 µL of whole blood (65% hematocrit) in 5 minutes using a single pipette.
Achieves 97% plasma recovery with 100% separation efficiency.
No significant difference in recovery rates for small molecules, proteins, and nucleic acid biomarkers compared to centrifugation.

Conclusions:

The developed method is a viable and excellent alternative to traditional plasma preparation.
Enables efficient and cost-effective plasma separation for point-of-care diagnostics.
Facilitates microfluidic applications in clinical settings and at the patient's bedside.