Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.5K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.5K
Forced Transdifferentiation01:28

Forced Transdifferentiation

2.2K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

17-DMAG-Loaded HER2-Targeted Extracellular Vesicles Induce PARP/Caspase3-Mediated Apoptosis in Gastric Carcinoma.

International journal of molecular sciences·2026
Same author

Efficacy of atopic dermatitis alleviation through transcutaneous auricular vagus nerve stimulation in 2,4-dinitrochlorobenzene (DNCB)-induced mouse model.

Annals of medicine·2026
Same author

Thermally Tunable Photonic Synaptic Transistor with Bioinspired Temporal Dynamics for Task-Adaptive Neuromorphic Vision.

ACS applied materials & interfaces·2026
Same author

Thiazole-Linked <i>N</i>-Hydroxypropanamide Derivatives: Selective HDAC6 Inhibitors with Therapeutic Potential for Neurodegenerative Diseases.

Journal of medicinal chemistry·2026
Same author

HER2-targeted doxorubicin-loaded cell-derived extracellular vesicles induce apoptosis of breast cancer cells via ROS/TXNIP pathway activation.

Cancer cell international·2026
Same author

Sero-Epidemiologic Study of Respiratory Syncytial Virus Infection in Korea.

Journal of Korean medical science·2026
Same journal

Taphonomic analysis at Liang Bua reveals the behavioral and technological capabilities of <i>Homo floresiensis</i>.

Science advances·2026
Same journal

Targeting granule initiation and amyloplast structure to create giant starch granules in wheat.

Science advances·2026
Same journal

A meta-analysis of carbon losses and gains from tropical moist forest degradation and regeneration.

Science advances·2026
Same journal

Ancient DNA reveals elite dynastic rule among Iron Age Eurasian Steppe nomads.

Science advances·2026
Same journal

Targeting astrocytic Dp71 attenuates BBB disruption after traumatic brain injury through WTAP-associated m<sup>6</sup>A regulation of MMP2.

Science advances·2026
Same journal

Pancreatic α cells are required for nutrient homeostasis by regulating dynamic β cell networks in islets.

Science advances·2026
See all related articles

Related Experiment Video

Updated: Dec 25, 2025

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

39.4K

Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes.

Youn Jae Jung1,2, Hark Kyun Kim3, Yoonsuk Cho3

  • 1Department of Materials Science and Chemical Engineering, Hanyang University ERICA, Ansan 15588, Republic of Korea.

Science Advances
|April 2, 2020
PubMed
Summary
This summary is machine-generated.

Stem cell-derived extracellular vesicles (EVs) show promise for regenerative medicine. These EVs can reprogram cells, offering a cell-free therapeutic approach for obesity and metabolic disorders.

More Related Videos

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.8K
Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
09:52

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

2.6K

Related Experiment Videos

Last Updated: Dec 25, 2025

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

39.4K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.8K
Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
09:52

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

2.6K

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biotechnology

Background:

  • Stem cell-derived extracellular vesicles (EVs) are explored as cell-free therapeutic systems.
  • EVs can mediate regenerative processes, offering an alternative to cell-based therapies.

Purpose of the Study:

  • To develop stem cell EVs derived during differentiation for tissue-specific therapeutic applications.
  • To investigate the potential of these EVs in treating obesity and related metabolic disorders.

Main Methods:

  • Extracellular vesicles (EVs) were isolated from human adipose-derived stem cells (HASCs) during white (D-EV) and beige (BD-EV) adipogenic differentiation using tangential flow filtration.
  • D-EV and BD-EV were used to differentiate HASCs in vitro and transplanted into mice models for in vivo studies.

Main Results:

  • D-EV and BD-EV successfully induced differentiation of HASCs into white and beige adipocytes, respectively.
  • Transplanted D-EV promoted lipid droplet formation in mice.
  • BD-EV treatment attenuated diet-induced obesity by promoting adipose tissue browning and improved hepatic steatosis and glucose tolerance in mice.
  • MicroRNAs (miRNAs) within BD-EV were identified as key mediators of these therapeutic effects.

Conclusions:

  • Secreted EVs during stem cell differentiation can act as potent cell-free therapeutic agents.
  • These EVs hold potential for reprogramming cells and treating metabolic diseases like obesity and hepatic steatosis.