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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Related Experiment Video

Updated: Dec 25, 2025

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
08:17

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Published on: September 22, 2017

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Imaging retinal structures at cellular-level resolution by visible-light optical coherence tomography.

Shaohua Pi, Tristan T Hormel, Xiang Wei

    Optics Letters
    |April 3, 2020
    PubMed
    Summary
    This summary is machine-generated.

    High-resolution optical coherence tomography enables cellular-level visualization of rat retinas in vivo. This breakthrough may reveal new biomarkers for diagnosing eye diseases.

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    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
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    Area of Science:

    • Ophthalmology
    • Biomedical Imaging
    • Neuroscience

    Background:

    • In vivo retinal imaging is crucial for understanding ocular function and disease.
    • Current techniques may lack the resolution to visualize cellular structures non-invasively.

    Purpose of the Study:

    • To develop and demonstrate a high-resolution in vivo retinal imaging technique.
    • To achieve cellular-level structural visualization of the entire retina in a rat model.

    Main Methods:

    • Utilized visible-light optical coherence tomography (OCT).
    • Employed volumetric registration and averaging for enhanced image quality.
    • Focused on imaging the complete retinal depth in rat eyes.

    Main Results:

    • Clearly visualized vitreous fibers, nerve fiber bundles, and vasculature.
    • Resolved at least three laminar sublayers within the inner plexiform layer.
    • Successfully imaged ganglion cell somas, inner nuclear layer cells, and photoreceptors.

    Conclusions:

    • This OCT technique offers unprecedented in vivo cellular resolution of the retina.
    • It represents a novel method for visualizing retinal microstructures.
    • Potential for detecting cellular neuronal biomarkers to aid in ocular disease diagnosis.