Related Experiment Video
Updated: Dec 25, 2025
06:35
Linearization of the Bradford Protein Assay
Published on: April 12, 2010
102.9K
Bradford Assay for Determining Protein Concentration.
Cold Spring Harbor Protocols
|April 3, 2020
Summary
The Bradford assay offers a rapid and sensitive method for determining protein concentrations. This technique relies on the color change of Coomassie Brilliant Blue G-250 dye upon binding to proteins.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Protein concentration determination is crucial in molecular biology and biochemistry.
- Accurate quantification of proteins is essential for various experimental procedures.
Purpose of the Study:
- To describe the Bradford assay as a method for protein quantification.
- To explain the underlying principle of the Bradford assay.
Main Methods:
- Utilizes the dye-binding properties of Coomassie Brilliant Blue G-250.
- Measures the shift in absorbance maximum of the dye.
Main Results:
- The dye's absorbance maximum shifts from 465 nm to 595 nm when bound to denatured proteins.
- This spectral shift allows for quantitative protein measurement.
Conclusions:
- The Bradford assay provides a quick and sensitive method for protein concentration measurement.
- The assay is based on a well-established spectrophotometric principle.
