Bradford Assay for Determining Protein Concentration
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Updated: Dec 25, 2025

06:35
Linearization of the Bradford Protein Assay
Published on: April 12, 2010
102.9K
Cold Spring Harbor Protocols
|April 3, 2020
Abstract:
The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

