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Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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2D NMR: Overview of Heteronuclear Correlation Techniques01:18

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Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
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Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
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In vivo comprehensive multiphase NMR.

Yalda Liaghati Mobarhan1, Ronald Soong1, Daniel Lane1

  • 1Environmental NMR Center, Department of Physical and Environmental Sciences, University of Toronto, Toronto, Ontario, Canada.

Magnetic Resonance in Chemistry : MRC
|April 3, 2020
PubMed
Summary
This summary is machine-generated.

Comprehensive multiphase (CMP) nuclear magnetic resonance (NMR) unifies solid and solution state hardware into a universal probe. This enables in vivo study of diverse biological phases, from liquids to solids, within intact organisms.

Keywords:
Comprehensive Multiphase NMRDaphnia magnaHyalella aztecaIn-vivoenvironmental organismsmagic angle spinningmetabolomics

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Area of Science:

  • Biophysical Chemistry
  • Magnetic Resonance Imaging
  • In Vivo Spectroscopy

Background:

  • Nuclear Magnetic Resonance (NMR) traditionally bifurcated into solid-state and solution-state techniques due to distinct hardware requirements.
  • This separation limited the comprehensive study of samples exhibiting multiple phases (liquid, gel-like, semisolid, solid).
  • Previous NMR applications in living systems were constrained by the inability to analyze the full spectrum of material properties simultaneously.

Purpose of the Study:

  • To introduce Comprehensive Multiphase (CMP) NMR as a unified approach for studying intact biological samples.
  • To provide a practical tutorial on applying in vivo CMP NMR for analyzing diverse biological phases within living organisms.
  • To highlight the potential of CMP NMR for gaining unique insights into the complex chemical environment of living systems.

Main Methods:

  • Development of a universal probe integrating all necessary hardware (magic angle spinning [MAS], gradients, RF handling, lock, susceptibility matching).
  • Application of CMP NMR to intact organisms, enabling the study of liquid, gel-like, semisolid, and solid phases.
  • Discussion of practical aspects including organism handling, rotor preparation, sample spinning, water suppression, and editing experiments.

Main Results:

  • CMP NMR successfully integrates diverse hardware, enabling comprehensive analysis of all sample phases within a single experimental setup.
  • In vivo application provides unprecedented insight into the range of molecular mobilities in living systems, from mobile fluids to rigid structures.
  • Demonstration of the adaptability of CMP NMR principles to conventional high-resolution MAS and solid-state NMR probes.

Conclusions:

  • CMP NMR overcomes the traditional hardware limitations, unifying solid and solution state NMR for comprehensive sample analysis.
  • This technology offers a powerful tool for in vivo research, revealing the intricate chemical landscape of living organisms across all phases.
  • The presented tutorial serves as a foundational resource for researchers adopting MAS-based approaches for in vivo studies.