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Updated: Dec 25, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
How to measure long-range proton-carbon coupling constants from 1 H-selective HSQMBC experiments
Josep Saurí1, Pau Nolis2, Teodor Parella2
1Structure Elucidation Group, Analytical Research and Development, Merck & Co., Inc., Boston, MA.
Abstract:
Heteronuclear long-range scalar coupling constants (n JCH ) are a valuable tool for solving problems in organic chemistry and are especially suited for stereochemical and configurational analyses of small molecules and natural products. This tutorial will focus on the step-by-step implementation of several 2D 1 H frequency selective HSQMBC experiments for the easy and accurate measurement of either the magnitude or both the magnitude and the sign of long-range n JCH couplings. The performance of these experiments will be showcased with several scenarios in a range of different experimental conditions.
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