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Related Concept Videos

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

2.1K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
2.1K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.7K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

7.7K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
7.7K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.7K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.7K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

6.6K
Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
6.6K

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Related Experiment Video

Updated: Mar 15, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

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The Evolving Landscape of NMR Structural Elucidation.

Josep Saurí1

  • 1Organic & Pharmaceutical Chemistry Department, Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Catalonia, Spain.

Molecules (Basel, Switzerland)
|March 14, 2026
PubMed
Summary

Nuclear Magnetic Resonance (NMR) spectroscopy advances structural elucidation. Modern NMR, enhanced by computation and new technologies, offers greater accuracy and efficiency in determining molecular structures.

Keywords:
NMR spectroscopystructure elucidation

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Structural Biology

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is a fundamental technique for molecular structure determination.
  • Traditional NMR methods have been enhanced by decades of technological and methodological progress.

Purpose of the Study:

  • To chart the evolution of NMR spectroscopy.
  • To highlight advancements in NMR capabilities and applications.
  • To showcase the synergy between NMR experiments and computational methods.

Main Methods:

  • Review of classical 1D/2D NMR experiments.
  • Integration of modern techniques: ultrahigh magnetic fields, cryogenic probes, non-uniform sampling, and hyperpolarization.
  • Application of computational tools: automated analysis, quantum chemical calculations, and machine learning.

Main Results:

  • Significant expansion of NMR capabilities and applications.
  • Enhanced accuracy and efficiency in molecular structure determination through experimental and computational synergy.
  • Broadened scope of NMR in analyzing complex mixtures, natural products, biomolecules, and materials.

Conclusions:

  • NMR spectroscopy continues to evolve as a powerful tool for structural elucidation.
  • The integration of advanced technologies and computational approaches is revolutionizing NMR.
  • NMR's applicability is expanding across diverse scientific disciplines.