Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

30.7K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
30.7K
Cell Signaling in Plants01:25

Cell Signaling in Plants

6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K
C4 Pathway and CAM01:27

C4 Pathway and CAM

48.4K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
48.4K
Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

27.7K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
27.7K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

7.7K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.7K
Morphogenesis02:19

Morphogenesis

30.0K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
30.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary <i>Artemisia ordosica</i> Krasch Supplementation Alters n-3 Polyunsaturated Fatty Acid Deposition and Lipid Metabolism in Cashmere Goat Meat.

Animals : an open access journal from MDPI·2026
Same author

Effects of <i>Artemisia ordosica Krasch</i>. aqueous extract on antioxidant and immune functions of broilers.

Frontiers in veterinary science·2026
Same author

Assembly-Independent Intramolecular Chiroptical Amplification in Figure-Eight-Shape Multi-Resonance Emitters: Efficient Solution-Processed Circularly Polarized Electroluminescence.

Angewandte Chemie (International ed. in English)·2026
Same author

Clay minerals alleviate lead contamination induced by soil speciation and oat translocation in neutral and saline soils.

Ecotoxicology and environmental safety·2026
Same author

The 2026 global roadmap for textile-integrated wearable technologies in health.

Physiological measurement·2026
Same author

Dynamic monitoring of circulating tumor cells and PD-L1 combined positive score as prognostic biomarkers for adjuvant immunochemotherapy in stage III gastric cancer: A prospective observational study.

International immunopharmacology·2026

Related Experiment Video

Updated: Dec 25, 2025

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.5K

The MAPK substrate MASS proteins regulate stomatal development in Arabidopsis.

Xueyi Xue1, Chao Bian1,2, Xiaoyu Guo1

  • 1The Waksman Institute of Microbiology, Rutgers, the State University of New Jersey; Piscataway, New Jersey, United States of America.

Plos Genetics
|April 3, 2020
PubMed
Summary

Mitogen-activated protein kinase (MAPK) signaling regulates plant stomatal development. New MAPK substrates in the stomatal lineage (MASS) proteins positively control stomatal production and patterning in Arabidopsis, interacting with key signaling components.

More Related Videos

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
05:22

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring

Published on: January 20, 2023

2.1K
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

10.2K

Related Experiment Videos

Last Updated: Dec 25, 2025

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.5K
Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
05:22

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring

Published on: January 20, 2023

2.1K
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

10.2K

Area of Science:

  • Plant Biology
  • Cell Signaling
  • Developmental Biology

Background:

  • Stomata are crucial for plant gas exchange and water regulation.
  • Mitogen-activated protein kinase (MAPK) signaling pathways influence stomatal development and patterning in plants.
  • Key MAPK components like MPK3/6 and transcription factors SPCH/SCRM are involved in stomatal formation regulation.

Purpose of the Study:

  • To functionally characterize the MAPK SUBSTRATES IN THE STOMATAL LINEAGE (MASS) proteins in Arabidopsis.
  • To investigate the role of MASS proteins in stomatal production and patterning.
  • To elucidate the interaction between MASS proteins and MAPK signaling components.

Main Methods:

  • Functional characterization of MASS proteins through genetic manipulation.
  • In vitro phosphorylation assays to determine MPK6 activity on MASS proteins.
  • Subcellular localization studies and domain analyses of MASS2.
  • Investigating potential interactions between MASS proteins and YODA (YDA).

Main Results:

  • MASS proteins are plasma membrane-associated positive regulators of stomatal production.
  • MPK6 phosphorylates MASS proteins, and mutations in substrate sites disrupt MASS localization and function.
  • Specific domains of MASS2 are critical for its localization and function.
  • MASS proteins may interact with YDA at the plasma membrane.

Conclusions:

  • MASS proteins are integral components of the MAPK signaling pathway in Arabidopsis stomatal development.
  • The YDA-MPK3/6 cascade, through MASS proteins, fine-tunes stomatal production and patterning.
  • This study reveals a functional divergence of the MAPK cascade in regulating plant development.