The spleen colony technique. II. Errors in estimation of the fraction of CFU-s synthesizing DNA
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Updated: Oct 24, 2026

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Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Published on: February 24, 2014
1Department of Pathophysiology, Faculty of Medicine, Charles University, Prague, Czechoslovakia.
Abstract:
A refinement of the method for determining the fraction of CFU-s in the S phase is presented. The new procedures were tested by means of a model experiment in which the fraction of CFU-s 'killed' was simulated by diluting the cell suspension. Another test was to determine the fraction of the CFU-s in the S phase using duplicate samples.

