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Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
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Differential Localization of Structural and Non-Structural Proteins during the Bluetongue Virus Replication Cycle
Bjorn-Patrick Mohl1, Adeline Kerviel1, Thomas Labadie1
1Department of Infection Biology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK.
Viruses
|April 5, 2020
Summary
Bluetongue virus (BTV) assembles in cytoplasmic viral inclusion bodies (VIBs). Outer capsid protein VP5 and non-structural protein NS3 are crucial for BTV maturation and egress from infected cells.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Reoviridae family viruses replicate in cytoplasmic viral factories.
- Bluetongue virus (BTV) forms virus inclusion bodies (VIBs) for replication and assembly.
- VIBs are sites for initial assembly of transcriptionally active virus-like particles.
Purpose of the Study:
- To characterize the formation, composition, and ultrastructure of BTV VIBs.
- To elucidate the role of viral proteins in VIBs and virus replication.
- To understand BTV maturation and egress mechanisms.
Main Methods:
- Microscopic techniques including structured illumination microscopy.
- Virological assays.
- Analysis of viral protein association with VIBs.
Main Results:
- Outer capsid protein VP5 is associated with VIBs and essential for virus maturation.
- VP5 addition to virus cores arrests transcription and facilitates maturation.
- Glycosylated non-structural protein NS3 shows time-dependent association with VIBs.
- Polybasic motifs in NS3 are important for virus trafficking and egress.
Conclusions:
- VP5 is integral to BTV VIBs and essential for arresting transcription and maturation.
- The dynamic association of NS3 with VIBs is critical for viral egress.
- This study provides novel insights into BTV VIB formation and virus replication processes.
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