Related Experiment Video
Updated: Dec 25, 2025
Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
Published on: June 14, 2016
Protective and predictive role of Mucin1 in sepsis-induced ALI/ARDS
Yu-Ming Wang1, Xing Qi1, Fang-Chen Gong1
1Department of Emergency in Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
Objective:
We aimed to investigate whether inhibition of MUC1 would aggravate sepsis-induced ALI, and explore the predictive value of plasma MUC1 for sepsis patients with or without ARDS.
Materials And Methods:
MUC1 siRNA pre-treatment was used to knockdown MUC1 expression in vitro. GO203 was used to inhibit the homodimerization of MUC1-C in vivo. Expression levels of MUC1, TLR 4 and HIF-1α were detected by Western blot. In addition, plasma MUC1 levels of enrolled patients were detected by ELISA on the day of admission and on the 3rd day. ROC curve was used to determine the predictive value of MUC1 in sepsis patients with ARDS.
Results:
Our results showed that inhibition of MUC1 could aggravate sepsis-induced acute lung injury and increase the expression of inflammatory cytokines in sera and BALF of sepsis mice. At the same time, we confirmed that inhibition of MUC1 could significantly decrease HIF-1α expression and thereby activate the expression level of TLR4. HIF-1α was a negative regulator of TLR-4. In addition, plasma MUC1 levels of sepsis patients with ARDS were significantly higher than those without ARDS and healthy adults. ROC curve showed that predictive value of plasma MUC1 on sepsis with ARDS on the 3rd day of enrollment was higher than the day of enrollment.
Conclusion:
MUC1 could inhibit the expression of TLR-4 by stabilizing HIF-1α, thereby alleviate sepsis-induced lung injury and protect organ function. At the same time, elevated MUC1 levels in plasma had a good predictive valud on whether patients with sepsis would develop ARDS.
Insights
Inhibition of MUC1 worsens sepsis-induced acute lung injury. However, elevated plasma MUC1 levels predict acute respiratory distress syndrome (ARDS) in sepsis patients, offering diagnostic value.
Area of Science:
- Biomedical research
- Molecular biology
- Critical care medicine
Background:
- Mucin 1 (MUC1) is implicated in cellular processes.
- Sepsis-induced acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are critical conditions.
- The role of MUC1 in sepsis-induced ALI and its predictive value for ARDS require clarification.
Purpose of the Study:
- To investigate if MUC1 inhibition exacerbates sepsis-induced ALI.
- To explore the predictive value of plasma MUC1 for sepsis patients with or without ARDS.
Main Methods:
- MUC1 expression was inhibited using MUC1 siRNA in vitro and GO203 in vivo.
- Western blot analysis detected MUC1, TLR4, and HIF-1α expression.
- Plasma MUC1 levels were measured by ELISA in sepsis patients and healthy controls.
- Receiver operating characteristic (ROC) curve analysis assessed MUC1's predictive value for ARDS.
Main Results:
- MUC1 inhibition aggravated sepsis-induced ALI and increased inflammatory cytokines.
- MUC1 inhibition decreased HIF-1α expression, leading to increased TLR4 activation.
- Plasma MUC1 levels were significantly higher in sepsis patients with ARDS compared to those without and healthy adults.
- Plasma MUC1 showed higher predictive value for ARDS on day 3 compared to day 1 of enrollment.
Conclusions:
- MUC1 inhibits TLR-4 expression by stabilizing HIF-1α, alleviating sepsis-induced lung injury.
- Elevated plasma MUC1 levels are a valuable predictor of ARDS development in sepsis patients.
Related Concept Videos
01:29Acute Respiratory Failure-V
Ensure that patients are monitored continuously for their response to therapy, including changes in...
01:24Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors