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Published on: January 21, 2015
Fiber attenuated total reflection infrared spectroscopy of kidney tissue during live surgery
Valdas Sablinskas1, Rimante Bandzeviciute1, Martynas Velicka1
1Institute of Chemical Physics, Vilnius University, Vilnius, Lithuania.
Abstract:
More than 90% of solid kidney tumors are cancerous and have to be treated by surgical resection where surgical outcomes and patient prognosis are dependent on the tumor discrimination. The development of alternative approaches based on a new generation of fiber attenuated total reflection (ATR) probes could aid tumor identification even under intrasurgical conditions. Herein, fiber ATR IR spectroscopy is employed to distinguish normal and cancerous kidney tissues. Freshly resected tissue samples from 34 patients are investigated under nearly native conditions. Spectral marker bands that allow a reliable discrimination between tumor and normal tissue are identified by a supervised classification algorithm. The absorbance values of the bands at 1025, 1155 and 1240 cm-1 assigned to glycogen and fructose 1,6-bisphosphatase are used as the clearest markers for the tissue discrimination. Absorbance threshold values for tumor and normal tissue are determined by discriminant analysis. This new approach allows the surgeon to make a clinical diagnosis.
Insights
Fiber attenuated total reflection (ATR) infrared spectroscopy can distinguish cancerous from normal kidney tissue. This method identifies specific spectral markers, aiding surgeons in real-time tumor discrimination during operations.
Area of Science:
- Biomedical Engineering
- Spectroscopy
- Oncology
Background:
- Accurate discrimination between cancerous and normal kidney tissue is crucial for successful surgical resection and patient prognosis.
- Current methods for intraoperative tumor identification can be challenging, necessitating advanced diagnostic tools.
Purpose of the Study:
- To investigate the utility of fiber attenuated total reflection (ATR) infrared spectroscopy for distinguishing cancerous from normal kidney tissues.
- To identify specific spectral markers indicative of kidney tumors for intraoperative use.
Main Methods:
- Fiber ATR IR spectroscopy was applied to freshly resected kidney tissue samples from 34 patients.
- A supervised classification algorithm was used to identify spectral marker bands for tissue discrimination.
- Discriminant analysis determined absorbance threshold values for classifying tissues.
Main Results:
- Specific spectral marker bands at 1025, 1155, and 1240 cm⁻¹ were identified as reliable indicators for differentiating tumor from normal kidney tissue.
- These bands are associated with glycogen and fructose 1,6-bisphosphatase.
- Absorbance threshold values were established for accurate tissue classification.
Conclusions:
- Fiber ATR IR spectroscopy offers a promising approach for real-time, intrasurgical identification of kidney tumors.
- This technique can assist surgeons in making clinical diagnoses, potentially improving surgical outcomes.
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