Chiral Cu_x OS@ZIF-8 Nanostructures for Ultrasensitive Quantification of Hydrogen Sulfide In Vivo

In this study, a Cu_x OS@ZIF-8 nanostructure is fabricated to quantify the levels of hydrogen sulfide (H₂ S) in living cells and in vivo. Zeolitic lmidazolate framework-8 (ZIF-8) is chosen as an encapsulation shell to improve the selectivity of this probe. Using this unique nanostructure, ultrasensitive quantification and bioimaging of H₂ S in living cells are successfully achieved. The lower limit of detection is 0.8 and 5.3 nmol per 10⁶ cells for circular dichroism and fluorescence modes, respectively. It is found that the chiral Cu_x OS NPs transformed into achiral Cu_x S NPs contribute to the ultrasensitive detection. Notably, this probe can also be carried out to detect and track H₂ S levels in tumor-bearing animals. The discoveries put forward for the creation of a detection platform for quantitative tracking and analysis in clinic.