Analysis of bisphosphonate using ultraviolet-visible spectroscopy based on modified phosphorus determination reagent
Jinyu Wang1, Yaping Wu1, Jiawei Jiang1
1Institution of Biomedicine and Tissue Engineering, School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu 225002, PR China.
Abstract:
An improved phosphorous determination was developed using ethanol, phosphorus determination reagent (PDR) and Ultraviolet-visible spectroscopy (UV-Vis) for analyzing the bisphosphonates (BPs). The method was carried out under mild conditions without digestion, high temperature, high pressure, and other extreme conditions. Alcohols played an important role in this method. Without alcohol, this reaction system did not have a color reaction. Alendronate (ALN) and risedronate (RIS) were used to demonstrate the reliability of the improved phosphorous determination under different reaction conditions. The absorbance had an equal ratio of increase as well as a good trend line when the content of BPs increased. The improved phosphorous determination could be a new method to measure the drug content of BPs.
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