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Fluorometric virus detection platform using quantum dots-gold nanocomposites optimizing the linker length variation.
Fahmida Nasrin1, Ankan Dutta Chowdhury2, Kenshin Takemura1
1Laboratory of Biotechnology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
Analytica Chimica Acta
|April 8, 2020
Summary
This study introduces a tunable biosensor for virus detection using quantum dots and gold nanoparticles. The novel biosensor enhances fluorescence and detects influenza virus with high sensitivity.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Localized Surface Plasmon Resonance (LSPR) biosensors offer high sensitivity for biomolecule detection.
- Quantum dots (QDs) and gold nanoparticles (AuNPs) are key components in advanced biosensing platforms.
- Controlling nanoparticle-to-nanoparticle distance is crucial for optimizing LSPR-based signal transduction.
Purpose of the Study:
- To develop a tunable biosensor for virus detection by precisely controlling the distance between CdZnSeS/ZnSeS quantum dots (QDs) and gold nanoparticles (AuNPs).
- To investigate the influence of peptide linkage length on the LSPR and fluorescence properties of QD-AuNP nanocomposites.
- To demonstrate the biosensor's capability for sensitive and specific detection of influenza virus.
Main Methods:
- Fabrication of a tunable biosensor by linking QDs and AuNPs with an 18-amino acid peptide chain.
- Utilizing LSPR to modulate the fluorescence of QDs based on their proximity to AuNPs.
- Inducing steric hindrance via virus binding to alter LSPR behavior and quench QD fluorescence.
- Characterization of CdZnSeS/ZnSeS QD-peptide-AuNP nanocomposites and their response to varying virus concentrations.
Main Results:
- Optimized QD-AuNP distance enhanced QD fluorescence through surface plasmon effects.
- Virus binding induced steric hindrance, leading to LSPR changes and QD fluorescence quenching.
- The biosensor successfully detected influenza virus across a concentration range of 10^-14 to 10^-9 g mL^-1.
- Achieved a highly sensitive detection limit of 17.02 fg mL^-1 for influenza virus.
Conclusions:
- The developed tunable biosensor demonstrates a promising approach for sensitive virus detection.
- The QD-peptide-AuNP nanocomposite system offers a versatile platform for infectious disease diagnostics.
- This LSPR-based biosensor presents a viable alternative for various virus detection applications.
