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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Dec 24, 2025

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
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A reduction-responsive liposomal nanocarrier with self-reporting ability for efficient gene delivery.

Bing Wang1, Ji Zhang, Yan-Hong Liu

  • 1Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, P. R. China. jzhang@scu.edu.cn xqyu@scu.edu.cn.

Journal of Materials Chemistry. B
|April 8, 2020
PubMed
Summary

Researchers developed a novel self-reporting liposomal nanocarrier (FNSL) for gene delivery. This FNSL nanocarrier monitors its reduction capability and enhances gene transfection efficiency with reduced toxicity.

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Gene Therapy

Background:

  • Developing reduction-responsive nanocarriers for gene delivery is challenging due to difficulties in monitoring their reduction capability, especially intracellularly.
  • Existing nanocarriers often lack self-reporting features to track their performance in real-time.

Purpose of the Study:

  • To develop a novel, reduction-sensitive, and self-reporting liposomal nanocarrier (FNSL) for enhanced gene delivery.
  • To utilize the naphthalimide-sulfonamide (NS) group's fluorescence signal for monitoring reduction and gene release.
  • To evaluate the FNSL nanocarrier's gene delivery efficiency, cytotoxicity, and responsiveness to glutathione (GSH).

Main Methods:

  • Synthesized a novel liposomal nanocarrier (FNSL) incorporating the naphthalimide-sulfonamide (NS) group for reduction sensitivity and fluorescence reporting.
  • Characterized FNSL's DNA binding, nanoparticle formation, size, and zeta potential.
  • Assessed FNSL's gene release capability, transfection efficiency (TE) in HepG2 cells, and cytotoxicity compared to a non-reducible control (FNAL).
  • Investigated the effect of exogenous glutathione (GSH) on FNSL's TE.

Main Results:

  • FNSL demonstrated reduction-sensitive property and self-reporting character, emitting green fluorescence (500 nm) upon reduction by GSH.
  • FNSL effectively condensed DNA into nanoparticles with suitable size and zeta potential.
  • FNSL exhibited enhanced gene release, higher TE, and lower cytotoxicity compared to FNAL.
  • Exogenous GSH significantly improved FNSL's TE in HepG2 cells, surpassing Lipofectamine 2000.

Conclusions:

  • FNSL is a promising bioreducible liposomal nanocarrier with self-reporting ability for efficient and low-toxicity gene delivery.
  • The fluorescence reporting mechanism allows convenient assessment of carrier reducibility and monitoring of stimuli-responsive gene release.
  • This study provides a valuable platform for developing advanced biodegradable gene delivery systems.