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Dopamine assisted PMOXA/PAA brushes for their switchable protein adsorption/desorption
Chao Pan1, Xiaoru Liu, Kai Gong
1CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China. wangyanm@ustc.edu.cn.
Journal of Materials Chemistry. B
|April 8, 2020
Summary
This study developed tunable protein-resistant surfaces using mixed polymer brushes. These surfaces allow controlled protein adsorption and desorption, crucial for biomedical applications.
Area of Science:
- Materials Science
- Biomaterials Engineering
- Surface Chemistry
Background:
- Protein adsorption on surfaces is a significant challenge in biological applications, often leading to uncontrolled and irreversible interactions.
- Developing surfaces with tunable protein resistance is critical for advancing biomaterials and medical devices.
Purpose of the Study:
- To create and characterize mixed polymer brushes with switchable protein adsorption/desorption properties.
- To investigate the influence of polymer composition and molecular weight on surface characteristics and protein interactions.
Main Methods:
- Sequential grafting of poly(2-methyl-2-oxazoline) (PMOXA) and poly(acrylic acid) (PAA) onto poly(dopamine)-coated substrates.
- Characterization using X-ray photoelectron spectroscopy (XPS), ellipsometry, and water contact angle (WCA) measurements.
- Monitoring protein adsorption and desorption via fluorescence microscopy and surface plasmon resonance (SPR).
Main Results:
- Mixed PMOXA/PAA brushes exhibited tunable surface wetting properties with changes in pH and ionic strength.
- Protein adsorption, specifically of bovine serum albumin (BSA), was effectively regulated by grafting time and PAA molecular weight.
- Up to 87% of adsorbed BSA could be desorbed by altering pH and ionic strength, demonstrating switchable behavior.
Conclusions:
- Mixed PMOXA/PAA brushes offer a promising platform for controlled protein adsorption and desorption.
- The combination of PMOXA's protein resistance and PAA's pH/salt sensitivity enables highly switchable surface functionalities.
- These findings have implications for designing advanced biomaterials with responsive protein interaction capabilities.
