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Related Experiment Video

Updated: Dec 24, 2025

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

A multi-responsive biomimetic nano-complex platform for enhanced gene delivery.

Xiaoyu Bai¹, Ming Kong, Xuanjin Wu

¹College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, P. R. China. kongming@ouc.edu.cn xgchen@ouc.edu.cn.

Journal of Materials Chemistry. B

|April 8, 2020

Summary

This study developed a novel nano-complex for enhanced siRNA delivery, overcoming barriers for RNA interference (RNAi) cancer therapy. The biomimetic system improved intracellular siRNA accumulation and gene silencing in cancer cells.

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Last Updated: Dec 24, 2025

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

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Published on: July 31, 2015

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

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Published on: March 1, 2013

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Area of Science:

Biomedical Engineering
Nanotechnology
Molecular Biology

Background:

RNA interference (RNAi) shows promise for disease treatment but faces delivery challenges.
Efficient siRNA delivery vehicles are needed to overcome extra- and intracellular barriers for clinical applications.

Purpose of the Study:

To develop a novel, multi-responsive nano-complex for enhanced siRNA delivery.
To improve intracellular siRNA accumulation and gene silencing efficacy for cancer therapy.

Main Methods:

Preparation of a nano-complex integrating thioglycolic acid conjugated chitosan nanoparticles (TCS NPs), transfersomes (T), and amphiphilic hyaluronic acid (HA-GMS).
Loading vascular endothelial growth factor (VEGF) siRNA into TCS NPs, cloaked by transfersomes with HA-GMS on the surface (HT-TCS-siRNA NPs).
Evaluation of nano-complex stability, pH-responsive release, proton sponge effect, in vitro cell uptake, and gene silencing in HeLa cells.

Main Results:

The HT-TCS-siRNA NPs demonstrated superior siRNA protection and stability at physiological and tumor-mimicking pH.
The nano-complex exhibited proton sponge effects at endo/lysosomal pH and GSH-triggered release in the cytoplasm.
Enhanced intracellular siRNA accumulation and VEGF gene silencing were observed in HeLa cells.

Conclusions:

The multi-responsive biomimetic nano-complex offers enhanced gene delivery capacity.
This novel system holds potential for effective application in cancer therapy via RNAi.