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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
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Cell Membrane-Specific Fluorescent Probe Featuring Dual and Aggregation-Induced Emissions.

Yibin Zhang, Yunnan Yan, Shuai Xia

    ACS Applied Materials & Interfaces
    |April 8, 2020
    PubMed
    Summary

    Researchers developed a novel fluorescent probe for cell membranes. This probe utilizes aggregation-induced emission (AIE) for enhanced imaging, showing strong fluorescence upon binding to cell membranes with low toxicity and high stability.

    Keywords:
    aggregation-induced emissioncell membranecellular imagingcoumarindual emissiontetraphenylethene

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    Area of Science:

    • Chemical Biology
    • Materials Science
    • Biotechnology

    Background:

    • Cell membrane imaging is crucial for understanding cellular processes.
    • Traditional fluorescent probes often face challenges like photobleaching and poor retention.
    • Aggregation-Induced Emission (AIE) probes offer unique advantages for bioimaging.

    Purpose of the Study:

    • To design and synthesize a novel cell membrane-specific fluorescent probe.
    • To investigate the probe's photophysical properties, including its AIE behavior.
    • To evaluate the probe's performance for cell membrane imaging in vitro.

    Main Methods:

    • Conjugation of a coumarin dye with a tetraphenylethene (TPE) derivative.
    • Characterization of spectral properties (absorption and emission) in different solvents.
    • Assessment of aggregation-induced emission (AIE) properties by varying water content.
    • Cellular imaging studies to evaluate membrane specificity, retention, and cytotoxicity.

    Main Results:

    • A novel fluorescent probe was successfully synthesized, combining coumarin and TPE moieties.
    • The probe exhibits dual absorption peaks and fluorescence emission with aggregation-induced emission (AIE) characteristics.
    • In aqueous solutions, the probe shows a significant red shift in fluorescence emission (470 nm to 591 nm) with increasing water content.
    • The probe effectively labels cell membranes, displaying strong fluorescence at 591 nm with long retention times and low cytotoxicity.
    • Excellent photostability was observed for the developed probe.

    Conclusions:

    • The developed coumarin-TPE based fluorescent probe is highly effective for cell membrane imaging.
    • Its unique AIE property and specific membrane binding enable robust and stable cellular visualization.
    • This probe offers a promising alternative to traditional membrane probes due to its enhanced performance and low toxicity.