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Amyloid precursor-like protein 2 interacts with claudin-7 and affects ovarian cancer cell survival
Neetu Dahiya1,2
1Laboratory of Molecular Biology & Immunology, NIH, Baltimore, MD 21224, USA.
Aim:
In our previous report, we identified roles of CLDN7 in regulation of cell signaling. The goal of this study was to identify proteins interacting with CLDN7 in ovarian cancer.
Methods:
The yeast two-hybrid system was used to identify proteins directly interacting with CLDN7 and cell survival was tested using colony formation assay.
Results:
Amyloid precursor-like protein 2 (APLP2) was found directly associated with CLDN7 in ovarian cancer cell line OVCA420. In addition, APLP2 showed increased expression in ovarian cancer cell lines and tumor tissue samples compared with non-neoplastic ovarian tissues. Knockdown of CLDN7 led to increased expression of APLP2 at both the mRNA and protein levels. Knockdown of APLP2 was associated with decreased cell survival in ovarian cancer cells.
Conclusion:
We show a direct interaction of CLDN7 with APLP2. These findings suggest novel regulatory role for APLP2 in ovarian cancer, a role that appears to be mediated by CLDN7.
Insights
This study identified Amyloid precursor-like protein 2 (APLP2) interacting with Claudin 7 (CLDN7) in ovarian cancer. APLP2 may play a regulatory role in ovarian cancer progression, mediated by CLDN7.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Previous research identified roles for Claudin 7 (CLDN7) in cell signaling.
- Understanding protein interactions is crucial for elucidating cancer mechanisms.
Purpose of the Study:
- To identify proteins that directly interact with CLDN7 in ovarian cancer.
- To investigate the functional significance of CLDN7 interactions in ovarian cancer.
Main Methods:
- Yeast two-hybrid system to identify direct protein interactors of CLDN7.
- Colony formation assay to assess cell survival.
- Quantitative analysis of APLP2 expression in ovarian cancer tissues and cell lines.
Main Results:
- Amyloid precursor-like protein 2 (APLP2) was identified as a direct interactor of CLDN7 in the OVCA420 ovarian cancer cell line.
- APLP2 expression was elevated in ovarian cancer cell lines and tumor tissues compared to non-neoplastic controls.
- CLDN7 knockdown resulted in increased APLP2 mRNA and protein levels, while APLP2 knockdown reduced ovarian cancer cell survival.
Conclusions:
- A direct interaction between CLDN7 and APLP2 in ovarian cancer was demonstrated.
- APLP2 exhibits a novel regulatory role in ovarian cancer, potentially mediated by CLDN7.
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