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Atropine Differentially Modulates ECM Production by Ocular Fibroblasts, and Its Ocular Surface Toxicity Is Blunted by

Martina Cristaldi1, Melania Olivieri1, Salvatore Pezzino1

  • 1Research Center, Sooft Italia SpA c/o Biologic Tower, University of Catania, 95123 Catania, Italy.

Biomedicines
|April 9, 2020
PubMed

Insights

Atropine affects eye tissues differently, strengthening the sclera and altering the choroid. Colostrum and fucosyl-lactose may protect against atropine

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • The mechanisms of atropine's efficacy in treating progressive myopia remain unclear.
  • This study investigates the roles of scleral and choroidal fibroblasts in myopia and atropine's function.

Purpose of the Study:

  • To elucidate the differential effects of atropine on scleral and choroidal fibroblasts.
  • To assess the protective potential of colostrum and fucosyl-lactose against atropine-induced corneal epithelial cell toxicity.

Main Methods:

  • In vitro Western blotting assessed extracellular matrix (ECM) production by fibroblasts treated with atropine or 7-methylxanthine.
  • MTT assays evaluated corneal epithelial cell survival following atropine treatment, with or without colostrum or fucosyl-lactose.

Main Results:

  • Atropine and 7-methylxanthine increased collagen I and fibronectin in scleral fibroblasts but decreased them in choroidal fibroblasts.
  • Corneal epithelial cells treated with atropine showed reduced viability, which was mitigated by colostrum or fucosyl-lactose.

Conclusions:

  • Atropine exhibits distinct actions in different ocular tissues, reinforcing the scleral ECM and increasing choroidal permeability.
  • Colostrum and fucosyl-lactose show promise in reducing chronic atropine treatment's toxicity in pediatric myopia management.
Keywords:
2-fucosyl-lactose7-methyl-xanthineAtropinechoroidcollagencolostrumfibroblastsmyopiasclera

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